Sign Up
You are not currently logged in. Please log in to CEUfast to enable the course progress and auto resume features.

Course Library

Wound Series Part 2c: Wound Bed Cleansing (FL INITIAL Autonomous Practice- Pharmacology)

1 Contact Hour
Only FL APRN's will receive credit for this course
CEUfast OwlGet one year unlimited nursing CEUs $39Sign up now
This course is only applicable for Florida nurse practitioners who need to meet the autonomous practice initial licensure requirement.
This peer reviewed course is applicable for the following professions:
Advanced Practice Registered Nurse (APRN)
This course will be updated or discontinued on or before Saturday, April 25, 2026

Nationally Accredited

CEUFast, Inc. is accredited as a provider of nursing continuing professional development by the American Nurses Credentialing Center's Commission on Accreditation. ANCC Provider number #P0274.


= 92% of participants will know the process of proper wound bed cleansing.


After completing this continuing education course, the participant will be able to meet the following objectives:

  1. Identify considerations for pain management prior to wound cleansing and debridement.
  2. Utilize the TIME mnemonic to assess the wound prior to cleansing.
  3. Describe wound debridement methods.
  4. Differentiate wound inflammation from infection.
  5. Determine when it is appropriate to use antimicrobial wound cleansing solutions.
CEUFast Inc. and the course planners for this educational activity do not have any relevant financial relationship(s) to disclose with ineligible companies whose primary business is producing, marketing, selling, re-selling, or distributing healthcare products used by or on patients.

Last Updated:
To earn of certificate of completion you have one of two options:
  1. Take test and pass with a score of at least 80%
  2. Reflect on practice impact by completing self-reflection, self-assessment and course evaluation.
    (NOTE: Some approval agencies and organizations require you to take a test and self reflection is NOT an option.)
Authors:    Linda J. Cowan (PHD, ARNP, FNP-BC, CWS) , Alyssa King (DNP, APRN, CPNP-PC, PMHNP-BC, CLC, CNE)

Introduction: Wound Bed Cleansing

This wound care course serves as a continuation of the course series, Wound Series 2b: Wound Care, but it also can be taken alone for those interested in refreshing their knowledge and skills of the process of proper wound bed cleansing. This course will go over pain management considerations prior to wound cleansing and debridement, a brief review of the TIME/TIMES mnemonic from the prior course (Wound Series 2b: Wound Care), a more in-depth breakdown of this mnemonic to emphasize common debridement methods and how to differentiate inflammation of a wound versus an active infection, and an explanation of how to determine when it is appropriate to utilize antimicrobial wound cleansing solutions versus less cytotoxic methods.

Pain Management Prior to Wound Cleansing and Debridement

The Joint Commission (Joint Commission for Accreditation of Health Care Organizations [JCAHO], 2017) lists pain management as a quality indicator for acute care settings. Basic pain management includes an accurate assessment and documentation of wound pain. While pain management may be addressed most in burn wound settings, it is increasingly addressed in other chronic wounds (and rightly so!), as especially prior to wound cleansing and dressing changes.

The gold standard for an accurate assessment of the patient's pain is a self-reported pain level. Whether a pain scale of 0 (no pain) to 10 (excruciating pain) is used, or smiling faces/grimacing faces, the pain level should be documented exactly as the patient reports it. The onset, location of the pain, and the quality of pain (stabbing, shooting, throbbing, sharp, dull, constant or intermittent, intensity) along with what factors relieve the pain or make the pain worse (walking, standing, elevating the leg, etc.) should be ascertained and documented. The pain effects on the patient and any pain management history and current pain regimen should also be determined. Also, determine the patient's pain management goals (Sibbald et al., 2021).

Worsening wound pain is one hallmark of a deteriorating wound. It may indicate infection than other observable signs such as edema, warmth, and erythema surrounding chronic wounds. Woo and Sibbald developed a Chronic Wound Associated Pain (WAP) model (Krasner, 2014) to address the patient, the cause, and the wound. This algorithm may help determine an evidence-based approach to wound pain management. Persistent chronic pain is highly individualized and requires highly specialized medical care. If wound-related pain is not well managed, patients should be referred to specialists to address their wound pain. Persistent or chronic pain itself may impair wound healing by causing vasoconstriction and decreasing perfusion, so every attempt should be made to address chronic wound pain (Sibbald et al., 2021).

Pain with wound cleansing and dressing changes may be managed by topical anesthetic products such as 2% or 4% topical Lidocaine Jelly or EMLA cream or systemic medications such as NSAIDs or opioids. Topical anesthetics (Lidocaine, Prilocaine, etc.) should be applied with enough time before debridement or dressing change for the medication to take effect (approximately 10 min). Similarly, the systemic pain medication should also be administered to allow enough time for pain relief prior to starting (at least 30 minutes).

Addressing the pain when an infection is suspected would include addressing the infection. Certain wound products may assist with pain management in specific types of wounds. Matrix applications using small intestine submucosa (SIS) technology (typically porcine) have successfully relieved some wound pain, particularly over donor sites from split-thickness skin grafts. Glycerin or silicone-based gel sheets and hydrogel sheets (cross-linked polyethylene oxide and water) such as Vigilon may be cool and soothing to partial thickness wounds such as second-degree burns silicone helps reduce scarring after wound closure. Be on the alert for new, different (especially worsening), or persistent pain associated with chronic wounds. Persistent pain in a neuropathic extremity with a chronic wound may be associated with osteomyelitis (Baranoski & Ayello, 2020).


As introduced in the previous course in this wound series, Wound Series Part 2b: Wound Care, the TIME mnemonic is valuable for individuals participating in wound care. This wound bed preparation concept describes a systematic approach to addressing the wound bed's specific needs in full-thickness open wounds (Sibbald et al., 2021; Doughty & McNichol, 2015).

Evidence-based literature describes this mnemonic, T-I-M-E, to help clinicians perform evidence-based wound care (Gupta et al., 2017).

T is for Tissue

Tissue in the wound bed should be described and documented. This includes the appearance (color, consistency) of wound bed tissue and the presence and amount of any non-viable or necrotic tissue, as well as any hypergranulating tissue, hypertrophic scarring, or foreign bodies in the wound bed. Necrotic or non-viable tissue (including biofilm if detected) should be removed as appropriate or addressed. Hypergranulating tissue should be treated.

I is for Infection

Signs of infection should be identified and documented. Determine if a critical infection is present. This wound may need systemic treatment versus non-critical chronic bacterial colonization that may require only local management by debridement and topical therapies.

M is for Moisture

Moisture in the wound bed should be managed. A balance of moisture is important for wound healing. Topical wound care, especially dressings, is used to prevent the wound bed from drying out while eliminating excessive moisture in the wound bed. When excessive moisture in the wound exceeds the dressing's capabilities, it often causes moisture damage to the skin surrounding the open wound (maceration). Dressing selection is most important for addressing this specific need of the wound bed.

E is for the Edge of the Wound

Attention should be given to the wound edges. Rounded, "rolled" wound edges prevent epidermal cell migration, which may impair wound closure. Tracking or tunneling in the wound bed and undermining (a 'lip' or ledge under the wound edge) may impair healing. Undermining is often caused by shearing forces upon the wound area. It is frequently seen in pressure ulcers around the sacral area when the patient repeatedly slips down while sitting up in the bed.

This third course in the wound series will now build upon and further define this TIME mnemonic to guide you in wound cleansing procedures.

Tissue: Removing Necrotic or Non-viable Tissue from the Wound Bed

"Cleansing" the wound should not only address washing or rinsing a wound but also includes debridement or addressing the removal of non-viable or devitalized (necrotic) tissue from the wound bed as part of the 1st step in the TIME approach to wound management (Gupta et al., 2017).

Types of cleansing or debridement approaches should be based on a holistic approach to wound management, including the specific condition(s) of the wound (and patient), the established goals of treatment, and patient preferences (if possible) (Sibbald et al., 2021).

Necrotic tissue is a breeding ground for bacteria. It impairs wound healing (e.g., impairs optimal cellular communication and proliferation and acts as a physiological barrier to new tissue deposition and wound contraction) (Willy, 2013; Maheswary et al., 2021; Sibbald et al., 2021). Removing this unhealthy tissue (debridement) may be achieved by a variety of means (Salisbury & Percival, 2018; Maheswary et al., 2021; Sibbald et al., 2021):

  1. Sharp debridement, either selective or non-selective (with a scalpel, scissors, curette)
  2. Surfactants
  3. Enzymatic (collagenase ointment or similar)
  4. Autolytic (promoting the body's enzymatic activities)
  5. Mechanical (rough friction or wet-to-dry)
  6. Irrigation (including surgical water jet)
  7. Biosurgical or larval debridement (medical maggots)

In almost all cases, necrotic tissue should be removed when safely possible. This removal includes (Maheswary et al., 2021):

  • Slough (white, yellow, grey "chicken fat" appearing tissue)
  • Fibrin (adherent, white-yellow, or grey fibrous non-viable tissue)
  • Eschar (typically thick, brown to black, "leathery" dead tissue)

There are a few exceptions to this rule. One important exception is in the case of intact, hard, black eschar such as on the foot's heel without any signs of infection. There is no way to determine the depth of tissue damage underneath this eschar. Removing it may expose the bone and predispose the patient to infection and osteomyelitis. "Intact" is the keyword here. If this eschar is dry, NOT soft, boggy, or fluctuant, and does not have any lifting at any of the wound edges or drainage, it may be beneficial to leave this eschar alone. Of course, pressure should be offloaded from the area, and the eschar should be kept clean and dry (Doughty & McNichol, 2015).

Another exception is dry eschar in a person with advanced lower extremity arterial disease. Cover the eschar with dry gauze for protection and (if not contraindicated) "paint" it daily with a small amount of 10% povidone-iodine solution (commonly known as Betadine solution, providing 1% available Iodine) and let it air dry thoroughly before applying dry gauze for protection and padding (Gupta et al., 2017). With this tissue in place, it may act as a protective "body bandage" – keeping bacteria and contaminants out of the wound. Under this eschar, the wound healing process may continue if other wound healing impediments are addressed (offloading pressure, adequate nutrition & blood flow, adequate immune function, and tissue perfusion). If the wound under the eschar follows a healing trajectory, the eschar may lift itself after several weeks and display newly epithelialized skin underneath. In some cases, the eschar may lift prematurely at an edge or start feeling boggy or fluctuant underneath the eschar and may start draining or showing signs of infection. It may be best to remove the eschar (Sibbald et al., 2020).

Common Debridement Methods

As stated above, typical debridement methods include sharp debridement, mechanical, autolytic, enzymatic, and larval debridement (Sibbald et al., 2020; Moya-Lopez et al., 2020).

Sharp Debridement

Sharp debridement is usually performed with a scalpel, curette, scissors, and forceps. This debridement may occur in a surgical suite by a surgeon or bedside by a physician, physician’s assistant (PA), advanced practice registered nurse (APRN), or certified wound specialist if trained, competent, and allowed by state board scope of practice limitations.

Enzymatic Debridement

Enzymatic debridement is typically achieved by applying an ointment or gel containing plant-based or other enzymes (such as collagenase) to the wound bed.

Mechanical Debridement

Mechanical debridement may be accomplished by wet-to-dry dressings (if these are the best method for this patient), by rubbing/friction while cleansing the wound, or by pulsed water jets & hydrotherapy (pulsed lavage, whirlpool).

Autolytic Debridement

Autolytic debridement occurs by promoting natural enzymes in wound fluid to degrade non-viable tissues – usually by placing an occlusive or semi-occlusive dressing over the non-viable tissue. Autolytic debridement may be accomplished by osmotic wound products such as medical-grade honey or gauze saturated with hypertonic sodium chloride. These products "draw" fluid from wound bed tissues into the wound product and loosen necrotic tissue (Sibbald et al., 2020).

Larval Debridement

Larval debridement is also known as "natural" or biological debridement. Larval debridement is performed by allowing sterile maggots to remove non-viable tissue (Sibbald et al., 2020; Moya-Lopez et al., 2020). Many clinicians are relatively unfamiliar with larval debridement, so we will explore this unique debridement and treatment methodology.

Maggots are some of the unsung heroes of wound healing. Maggot or larval debridement therapy (MDT or LDT) has been utilized for medical purposes for hundreds or thousands of years (Sibbald et al., 2020; Moya-Lopez et al., 2020). Mayan Native Americans and other ancient cultures have documented reports of maggots being used in certain medical treatments, especially for wound care. Larvae of certain fly species, such as Lucilia sericata (green bottle fly), remove only dead tissue while promoting healthy tissue in the wound bed, helping wounds heal faster (Cowan et al., 2013; Moya-Lopez et al., 2020).

Regrettably, with the development of antibiotics in the 1940s and various skin and wound antiseptics, the use of LDT declined (Cowan et al., 2013; Moya-Lopez et al., 2020). It became a standard of care to use antibiotics in and on the body and antiseptics on the skin and the wound. Maggot therapy was abandoned in favor of more modern or "easier" methods. Arguably, one of the biggest reasons LDT may have lost favor in clinician's eyes was not ineffectiveness (for they remain a most effective form of debridement) but rather was the "yuck factor" - patients, their caregivers, and clinicians found it distasteful to apply small squirming worms that could crawl out of a wound.

Fortunately, with the advent of antibiotic-resistant organisms and increasing drug sensitivities, there was a renewed interest in the "natural" aspect of maggot therapy in the 1980s. The Food and Drug Administration (FDA) cleared medicinal maggots (Phaenicia or Lucilia sericata) for debriding non-healing necrotic skin and soft tissue wounds including (Cowan et al., 2013; Moya-Lopez et al., 2020):

  • Diabetic foot ulcers
  • Pressure ulcers
  • Non-healing surgical or traumatic wounds
  • Venous stasis ulcers

Larval therapy with maggots is classified as a medical device in the United States. However, maggots are classified as medicinal drugs in Europe, Canada, and Japan. Maggots used in the United States for larval debridement therapy are processed under controlled laboratory conditions. They are sterile (both free of disease and unable to reproduce). Larval debridement of non-viable tissue within chronic wounds results partly from the proteolytic digestive enzymes liquefying the necrotic tissue. The larvae then suck and remove bacteria and biofilm from the wound bed. As such, they are the most efficient way to debride a wound without the typical pain or bleeding associated with other forms of debridement, such as with sharp debridement (some patients with very sensitive wound beds may still experience some discomfort). Some bleeding precautions with larval debridement exists, however, such as not placing maggots over opening exposed artery/blood vessel. Research suggests they may be helpful in chronic wound refractory to other forms of treatment or debridement methods and effectively eliminate drug-resistant organisms such as MRSA (methicillin-resistant staphylococcus aureus) (King, 2020; Moya-Lopez et al., 2020; Romeyke, 2021; Siavash et al., 2021).

Is the Wound Infected?

Infection vs. Inflammation

In many cases, wound inflammation is often mistaken for wound infection.

The signs of inflammation are:

  • Warmth of wound
  • Edema around injury
  • Pain
  • Localized redness with well-defined borders

Patients with these symptoms are often placed on antimicrobial treatment regimens without the benefit of microbiological studies to guide them (no quantitative tissue cultures from the wound) (Weir & Schultz, 2016). This may be one contributing factor in the development of drug-resistant organisms.

Nevertheless, it is one of the most requested topics in basic wound care courses (to know when to treat and when not to treat "infection"). Therefore, we will quickly summarize some important facts and helpful tips regarding wound infection:

  • There is a difference between chronic bacterial colonization, critical colonization, and critical infection in a wound.
  • Chronic bacterial colonization does occur in most chronic wounds. If the bioburden (high levels and certain types of bacteria) in the wound is not high enough to impair wound healing and does not invade surrounding tissue, the wound may close when other comorbid conditions are addressed (such as nutrition), even if the bacteria are not removed or eliminated by the clinician. These cases of chronic colonization do not typically require antimicrobial intervention. In most of these cases, there may be persistent wound exudates. Still, typically these wounds do NOT exhibit worsening pain, worsening edema, or spreading erythema (redness) beyond 1cm from wound edges.
  • Alternatively, any open wound which is not improving AND has worsening symptoms of pain, worsening edema, or spreading warmth or erythema, should be evaluated for critical colonization (bacterial load is starting to penetrate surrounding tissue or impair wound healing). Critical colonization or infection is characterized by a high bioburden, which requires intervention (Weir & Schultz, 2016).
  • Critical colonization or infection may be determined by quantitative tissue culture (gold standard) typically obtained by a collecting small piece of tissue from the wound bed (usually a 3mm punch biopsy, a thorough curette scraping, a needle biopsy, or a small 2-4mm size tissue wedge removed by scalpel and pick-ups). Also, wound fluid may be aspirated and placed in a culture medium tube for wound culture. Any organism identified in greater than 105 or 100,000 (10x10x10x10x10) colony-forming units (CFUs) per gram of tissue or milliliter of wound fluid within the wound could be considered a critical infection requiring intervention.
  • Certain organisms are highly virulent (such as beta-hemolytic streptococcus). They will require intervention even if found in small amounts within the wound bed.
  • Suppose your facility does not perform quantitative culture analysis but reports bacterial growth in a semi-quantitative (1+, 2+, 3+, or 4+) scale. In that case, this may be equated to quantitative measures by considering 3+ may be close to 105 CFU, but 4+ is more than 105 CFU and considered a critical infection requiring intervention.
  • Some experts have pointed out that host immunity + virulence of organism + environmental factors + bacterial load are factors to consider when evaluating critical colonization and infection versus chronic colonization, which would not require intervention. Infection represents an imbalance in these factors, which results in impaired wound healing or invasion of bacterial organisms into the surrounding tissue.
  • Any wound that fails to progress for two weeks or more should be evaluated for high bioburden, considering that immune-compromised individuals may not display any classic signs or symptoms of infection (Zhao et al., 2016):
    • Warmth/systemic fever
    • Edema
    • Persistent pain
    • Redness that typically spreads edges diffuse and indistinct

Wound Cultures

Most nurses only collect wound surface swab cultures and do not typically collect tissue samples from the wound bed. However, there is much variation among swab culture techniques, which may significantly alter results.

Swab cultures of the wound bed surface immediately after removing a dressing may contain dressing contaminants and mostly loose free-floating bacteria. They may not represent what is actually in the wound bed. Also, necrotic tissue in the wound bed may influence culture results (Weir & Schultz, 2016). Wounds with non-viable tissue in the wound bed should be debrided before swab cultures whenever possible.

How the swab culture is collected is extremely important to an accurate diagnosis (Weir & Schultz, 2016):

  • Is the swab just lightly rubbed along the surface of the one with one stroke in one direction?
  • Is it collected with a back-and-forth motion across all surface areas of the wound with moderate downward pressure?
  • Is it rinsed with sterile saline first?

These questions should be resolved with standard evidence-based wound culture collection protocols, on which all clinicians can agree. Experts recommend a 3 or 4mm punch biopsy tissue sample for quantitative culture (Weir & Schultz, 2016). This is performed most often by trained physicians and nurse practitioners/physician assistants within their scope of practice and organizational policy. Alternates include using a curette scraping of the wound bed for quantitative culture. In some cases, a registered nurse who is board certified in wound care or another healthcare provider may do this within their scope of practice. Otherwise, a modified Levine technique may be utilized if swab cultures are the only option for culturing a wound (Weir & Schultz, 2016).

Levine Technique

A swab culture-collecting technique exists for when a quantitative tissue sample is not an option. This is the Levine Technique. The steps include (Sibbald et al., 2021):

  1. Rinse the wound bed once with sterile saline (non-bacteriostatic and preservative-free).
  2. Moisten the sterile culture swab with sterile non-bacteriostatic and preservative-free saline.
  3. Rotate the swab completely over a 1cm2 area in the wound bed where it is free of necrotic tissue.
  4. Press down slightly to illicit fresh wound fluid.
  5. Take your time to allow adequate time for fluid collection.
  6. Place in the appropriate culture media transport tube and ensure that the sample does not sit at room temperature longer than the lab recommends.
  7. Note: A wound culture left on the desk overnight will likely need to be repeated. Check your lab's policy on this!

The presence of biofilm (a protected colony of micro-organisms within the wound) is increasingly becoming associated with non-healing wounds. Wounds that have a biofilm often display impaired healing. Biofilms are not detectable to the naked eye and often overlook traditional swab culture laboratory techniques. They are resistant to topical as well as systemic antimicrobial treatments. Frequent debridement may be one way to reduce the presence of biofilm in wounds (sharp debridement, ultrasonic debridement, maggot debridement, etc.) (Sibbald et al., 2021).

Clinical Pearl

Osteomyelitis (infection in the bone) is associated with chronic, non-healing wounds. A very sensitive and specific diagnostic indicator of osteomyelitis (especially in diabetics) is when the clinician can see a bone or can "probe to the bone" (PTB) with a sterile cotton-tipped applicator or probe within the open wound (or at the end of wound tunneling). Other diagnostic tools to help evaluate possible osteomyelitis are (Doughty & McNichol, 2015):

  • Bone biopsy
  • Magnetic resonance imaging (MRI)
  • Bone scan

It is more appropriate to "rule out" osteomyelitis rather than "rule in" or positively diagnose osteomyelitis (Wier & Schultz, 2015).

Moisture Control

One of the main functions of a wound dressing or wound therapy is moisture management. Specifically, maintaining a moist wound bed while also eliminating excessive wound drainage. Since George Winter's seminal work in 1962 (Winter, 1962) demonstrated open wounds treated with dressings that maintained a moist environment healed almost 50% faster than wounds allowed to dry out, no published studies have been able to refute the effectiveness of moist wound healing. Current scientific investigations not only support what has been known about moist wound healing but serve to explain further the role of a moist wound bed in relation to the local cellular activity associated with wound healing (cytokine signaling, fibroblast cell proliferation, collagen and matrix synthesis, epithelial cell migration, etc.) and preventing additional trauma from wounding bed during dressing changes (Ermer-Seltun & Rolstad, 2016; Jaszarowski & Murphree, 2016).

Edge Management

Assessment (and documentation of the wound edges) is important for wound care. Rounded, "rolled" wound edges can prevent epidermal cell migration, ultimately impairing wound contraction and closure. Tracking or tunneling in the wound bed and undermining (a 'lip' or ledge under the wound edge) may also impair healing. Undermining is often caused by shearing forces upon the wound area, frequently seen in pressure ulcers around the sacral area, such as when the patient is repeatedly sliding down while attempting to sit up in the bed (Sibbald, 2021). In these cases, the potential cause needs to be considered (such as shearing forces) and eliminated where possible. In the case of a "rolled" wound edge, it may be necessary for surgical intervention or chemical cauterization (such as with silver nitrate sticks) by a qualified specialist.

Surrounding Skin and Tissues

The condition of the skin and tissue surrounding the open wound should be examined and palpated for (Gupta et al., 2017):

  • Pain/tenderness
  • Induration
  • Unusual warmth or coolness (different from the skin on the same extremity at a different location)
  • Maceration
  • New or satellite areas of erythema
  • Rash
  • Dryness
  • Scaling
  • Thickening
  • Bogginess
  • Crepitus
  • Bruising
  • Skin lightness or darkness compared to patient's skin tone
  • Blistering
  • Hyperkeratotic tissue

These findings should be reported/documented and monitored at each wound care visit (Gupta et al., 2017).

Cytotoxic Wound Cleansers

Now that you have completely pre-medicated for pain, completed a full wound assessment for inflammation versus infection, and collected any necessary tissue samples, you are ready to clean/debride the wound before dressing it.

Many experts agree that it is necessary to cleanse wounds at each dressing change to remove wound exudates, cellular waste, debris, bacteria, etc. (Doughty & McNichol, 2015). However, caution is warranted regarding the use of antimicrobial wound cleansers.

Cytotoxicity relates to a substance being toxic to human tissue cells. Cytotoxic wound cleansers will kill germs and kill healthy cells such as fibroblasts (white blood cells that manufacture collagen/new tissue) or epidermal keratinocytes (skin cells) (Doughty & McNichol, 2015). Also, many of these cleansers are antimitotic (they actually prevent cellular mitosis and regeneration), which is how some chemotherapy agents function to prevent cancer cell growth. While that may be a desirable trait for cancer treatment, it may not be desirable for a wound cleanser!

In most cases, wound experts recommend using water or saline to cleanse the wound. Some articles suggest potable tap water may be appropriate to cleanse a chronic wounds (Sibbald et al., 2021). However, it has been concluded that the decision to use tap water to cleanse wounds should consider the quality of water, nature of wounds, and the patient's general condition, including the presence of comorbid conditions (Gupta et al., 2017; Doughty & McNichol, 2015). Water must be potable for tap water to be considered for wound cleansing. Caution may be warranted for several reasons when considering tap water. It is not known if water used for wound cleansing vs. normal saline was tested for bacteria, cysts, fungi, or the presence of any other contaminants. If obtained from a well, tap water may contain contaminants such as bacteria or other contaminants undetectable to the human eye. Alternatively, "city water" is typically chlorinated at around three parts per million or 0.3mg/L and also typically contains fluoride (0.5mg/L to 1.0mg/L). Therefore, it is unknown what effect this may have on cellular activity in the wound bed (Gupta et al., 2017; Doughty & McNichol, 2015).

An exception to using cytotoxic wound cleansers is when the benefits outweigh the risks. For instance, in the case of localized bacterial invasion in which the host is unable to overcome the bioburden of the infecting organism(s) with its immune defenses (such as pseudomonas aeruginosa infection resulting in further wound deterioration), the fibroblasts and epidermal keratinocytes are not likely to survive this hostile wound environment anyway. Therefore, it may justify using a short-term application of cytotoxic wound cleanser, such as a dilute Dakin's Solution (sodium hypochlorite) long enough to eradicate the infecting organisms (Gupta et al., 2017; Doughty & McNichol, 2015). Yes, this is likely to impair cellular function temporarily. Still, the infecting organisms would do far worse if you did not address/control them. Once the wound is "cleaned up" (perhaps for even a few weeks), you should return to non-cytotoxic moist wound healing principles for wound care.

Dakin's Solution

Dakin’s Solution is a long-used cleansing solution that is used to kill bacteria and prevent bacterial growth in wounds (Keyes et al., 2022). It is a dilute sodium hypochlorite (NaClO) solution, more commonly referred to as “bleach” (Keyes et al., 2022). The main active ingredient in Dakin’s Solution is created when the chlorine in the solution reacts with water to form hypochlorous acid (HClO) (Keyes et al., 2022). It is this hypochlorous acid that produces an antibacterial effect in tissues (Keyes et al., 2022).

Ohio State University Medical Center (OSUMC) Department of Inpatient Nursing published a nice patient education pamphlet, "How to Make Dakin's Solution," available here.(Ohio State University Medical Center [OSUMC], 2002).

This pamphlet describes diluting 3 ounces of common (unscented) household bleach (5.25% sodium hypochlorite solution) with 32 ounces of clean, boiled water (buffered with ½ teaspoon baking soda/sodium bicarbonate) to make a full-strength Dakin's Solution (0.5% sodium hypochlorite solution).

To make ½ strength Dakin's Solution (0.25% sodium hypochlorite), use only 3 tablespoons (48ml) bleach mixed with the same amount of water (32 ounces) and baking soda (1/2 tsp). OSUMC reports tightly sealed jars of these solutions may be stored at room temperature for up to one month (in a dark jar), but once opened, any unused solution should be discarded within 48 hours.

Many clinicians are misinformed about Dakin's solution, specifically regarding when to use and not to use it, solution storage, documentation, and cytotoxic versus non-cytotoxic strengths. Here are some things every clinician should know about Dakin's:

  1. As described above, full strength Dakin's Solution is a 0.5% sodium chlorite (hypochlorous acid) solution. Most are buffered with sodium bicarbonate to bring the pH up to one more easily tolerated by human tissues.
  2. Dakin's Solution is cytotoxic at full strength, half strength, quarter strength (0.125% sodium hypochlorite), and even one-eighth strength (0.0625% sodium hypochlorite) fibroblasts and keratinocytes. Full strength Dakin's Solution would need to be diluted 50 times (one-fiftieth dilution) to reach a 0.01% sodium hypochlorite solution (a non-cytotoxic strength).
  3. Clinicians should ask:
    1. What strength was ordered?
    2. Is this strength appropriate?
    3. Is it ordered short-term or long-term? (Short-term use of quarter-strength Dakin’s may be appropriate to address a high localized bioburden because the benefits may outweigh the risks).
  4. Documentation of the use of any sodium hypochlorite solution should always be done by recording the exact strength used. Many times, all strengths are just recorded as "Dakin's Solution," but this is, in effect, the same as documenting that insulin was given without recording the exact strength. Dakin's Solution is a 0.5% sodium hypochlorite solution and is often diluted because of its high cytotoxic properties. Make sure to RECORD the correct strength!!!

A chart called "Know the Code" describes the different dilutions of sodium hypochlorite solution is available here.(Century Pharmaceuticals, n.d.).

Common side effects of Dakin’s Solution are necessary to know for patient safety. These side effects include swelling, redness, and local skin irritation (Keyes et al., 2022). Additional concerns regarding Dakin’s include skin hypersensitivity, allergic reactions, and impaired wound healing (Keyes et al., 2022). If there appears to be an adverse reaction occurring after the introduction of Dakin’s Solution, it should be discontinued immediately (Keyes et al., 2022).


This course covered pain management considerations prior to wound cleansing and debridement, a brief review of the TIME mnemonic, a more in-depth breakdown of this mnemonic to emphasize common debridement methods and how to differentiate inflammation of a wound versus an active infection, and an explanation of how to determine when it is appropriate to utilize antimicrobial wound cleansing solutions versus less cytotoxic methods. For more information regarding wound dressings, please continue forth within the wound care series to Wound Series Part 2d: Wound Dressings.

Select one of the following methods to complete this course.

Take TestPass an exam testing your knowledge of the course material.
No TestDescribe how this course will impact your practice.

Implicit Bias Statement

CEUFast, Inc. is committed to furthering diversity, equity, and inclusion (DEI). While reflecting on this course content, CEUFast, Inc. would like you to consider your individual perspective and question your own biases. Remember, implicit bias is a form of bias that impacts our practice as healthcare professionals. Implicit bias occurs when we have automatic prejudices, judgments, and/or a general attitude towards a person or a group of people based on associated stereotypes we have formed over time. These automatic thoughts occur without our conscious knowledge and without our intentional desire to discriminate. The concern with implicit bias is that this can impact our actions and decisions with our workplace leadership, colleagues, and even our patients. While it is our universal goal to treat everyone equally, our implicit biases can influence our interactions, assessments, communication, prioritization, and decision-making concerning patients, which can ultimately adversely impact health outcomes. It is important to keep this in mind in order to intentionally work to self-identify our own risk areas where our implicit biases might influence our behaviors. Together, we can cease perpetuating stereotypes and remind each other to remain mindful to help avoid reacting according to biases that are contrary to our conscious beliefs and values.


  • Baranoski, S. & Ayello, E. (2020). Wound Care Essentials: Practice Principles. 5th ed. Philadelphia, PA: Wolters Kluwer. Visit Source.
  • Century Pharmaceuticals. (n.d.). Know the code. Visit Source.
  • Doughty, D., McNichol, L. (2015). Wound Ostomy Continence Nurses Society Core Curriculum: Wound Management. Philadelphia, PA: Wolters Kluwer. Visit Source.
  • Gupta, S., Andersen, C., Black, J., de Leon, J., Fife, C., Lantis Ii, J. C., Niezgoda, J., Snyder, R., Sumpio, B., Tettelbach, W., Treadwell, T., Weir, D., & Silverman, R. P. (2017). Management of chronic wounds: Diagnosis, preparation, treatment, and follow-up. Wounds: A compendium of clinical research and practice, 29(9), S19–S36. Visit Source.
  • Joint Commission for Accreditation of Health Care Organizations (JCAHO). (2017). R3 Report: Pain assessment and management standards for hospitals. Issue 11. Retrieved October 22, 2021. Visit Source.
  • Keyes, M., Jamal, Z., & Thibodeau, R. (2022). Dakin solution. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing. Visit Source.
  • King, C. (2020). Changing attitudes toward maggot debridement therapy in wound treatment: a review and discussion. Journal of Wound Care, 29(Sup2c), S28–S34. Visit Source.
  • Krasner, D. (2014). Chronic Wound Care: The Essentials. Malvern, PA: HMP Communications. Visit Source.
  • Maheswary, T., Nurul, A. A., & Fauzi, M. B. (2021). The insights of microbes' roles in wound healing: A comprehensive review. Pharmaceutics, 13(7), 981. Visit Source.
  • Moya-López, J., Costela-Ruiz, V., García-Recio, E., Sherman, R. A., & De Luna-Bertos, E. (2020). Advantages of Maggot Debridement Therapy for Chronic Wounds: A Bibliographic Review. Advances in skin & wound care, 33(10), 515–525. Visit Source.
  • Ohio State University Medical Center. (OSUMC). (2002). How to make Dakin’s solution. Visit Source.
  • Romeyke, T. (2021). Maggot therapy as a part of a holistic approach in the treatment of multimorbid patients with chronic ulcer. Clinics and Practice, 11(2), 347–357. Visit Source.
  • Salisbury, A.M., Percival, S. (2018). Efficacy of a surfactant-based wound dressing in the prevention of biofilms. Adv Skin & Wound Care, 31(11), 514-520. Visit Source.
  • Siavash, M., Najjarnezhad, A., Mohseni, N., Abtahi, S. M., Karimy, A., & Sabzevari, M. H. (2021). Efficacy of maggot debridement therapy on refractory atypical diabetic foot ulcers: An open-label study. The International Journal of Lower Extremity Wounds, 20(4), 315–320. Visit Source.
  • Sibbald, R. G., Elliott, J. A., Persaud-Jaimangal, R., Goodman, L., Armstrong, D. G., Harley, C., Coelho, S., Xi, N., Evans, R., Mayer, D. O., Zhao, X., Heil, J., Kotru, B., Delmore, B., LeBlanc, K., Ayello, E. A., Smart, H., Tariq, G., Alavi, A., & Somayaji, R. (2021). Wound Bed Preparation 2021. Advances in Skin & Wound Care, 34(4), 183–195. Visit Source.
  • Willy, C. (2013). Antiseptics in surgery- Update 2013 scientific basis, indications for use. Visit Source.
  • Winter, G. D. (1962). Formation of the scab and the rate of epithelization of superficial wounds in the skin of the young domestic pig. Nature, 193, 293–294. Visit Source.